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Archive Number 20100208.0430
Published Date 08-FEB-2010
Subject PRO/AH/EDR> Botulism, canine - USA: (FL) susp.
BOTULISM, CANINE - USA: (FLORIDA) SUSPECTED
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International Society for Infectious Diseases
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Date: 5 Feb 2010
Source: The Miami Herald [edited]
<http://www.miamiherald.com/news/breaking-news/story/1464427.html>


A month after frigid temperatures killed reptiles all over South 
Florida, veterinarians are still treating dogs that may have 
contracted botulism by gnawing iguana carcasses. The result: 
paralysis so severe that a few had to be euthanized.

Like other cold-blooded creatures, thousands of iguanas froze to 
death during the freeze. Some dogs mistaking them for chew toys have 
shown classic symptoms of a disease so rare that most vets don't see 
a case for a decade. "I think it's botulism, although it's nearly 
impossible to prove definitively,'' said Dr. James Dugan, a Pinecrest 
vet. His clinic has treated several dogs suffering varying degrees of 
paralysis since the cold snap. All had munched on iguanas. "Why they 
want to eat a rotten reptile that smells horrible and could kill you, 
I don't know," Dugan said.

Paralysis begins in the back end, then progresses to the front legs, 
and in some cases disables the diaphragm. At that point, dogs must be 
intubated so they can breathe, an expensive step that many owners 
can't afford. In at least 2 such South Florida cases, dogs that 
couldn't breathe were euthanized. Serious problems like pneumonia 
also arise when paralysis affects the esophagus.

Broward County veterinary neurologist Dr. Brian Roberts first raised 
the alarm about the possible botulism link in a mass e-mail to South 
Florida colleagues in late January 2010. He said that several dogs 
had come to Veterinary Specialists of South Florida in Cooper City 
with paralyzed hindquarters. The common denominator: dead iguanas.

"We didn't have a clue what it was for days or weeks," said Roberts, 
who has sent tissue samples to the state's animal diagnostic 
laboratory in Kissimmee. He diagnosed botulism after ruling out other 
possible conditions. There's not much a vet can do beyond "supportive 
care and range-of-motion" exercises, he added.

Typically, paralysis sets in a day or so after the iguana encounter, 
and the worst symptoms last at least a week. The dogs can eat but 
can't stand or walk. However, they're not in pain, vets say.

That's the case with Baby Miller, a 68-pound, 8-year-old Pompano 
Beach pit bull whose human family rushed her to Dr. Kevin 
McAllister's clinic on 26 Jan 2010. "She picked up a dead iguana in 
the yard and by the next day was showing signs of weakness in the 
back leg that progressed to the front, and she could no longer 
stand," McAllister said. "She was in a little distress: high heart 
rate, anxious and totally down." The family "had no idea what it was, 
and I didn't either," said McAllister, in practice for 10 years. "We 
were thinking maybe a stroke or [human] drugs. In hindsight, she had 
the classic signs of botulism. I'd never seen it."

Baby's [owner] said that Baby had "gotten hold of an iguana" 2 days 
before she showed symptoms. "My brother found her on the floor, and 
she couldn't move," said the owner. Blood tests ruled out painkillers 
and roofing sealer, 2 possible culprits. The normally playful Baby is 
slowly recovering. "She is more irritated than anything. She's very 
aware of what's going on. She can lift her head and wag her tail and 
is trying to sit up. She has "refused to go to the bathroom in the 
house," and must be carried outside. The tab so far: USD 300 at the 
vet; USD 6 a day for Pedialyte to keep Baby hydrated. "She's been 
stable and didn't get any worse, and that's what we look for," McAllister said.

Dr. Jonathan Kreissler of Miami Veterinary Specialists said his 
hospital has seen "5 similar cases in 3 weeks. Three are not walking; 
one was euthanized," and one didn't return for follow-up care. Its 
owners can't be reached, and Kreissler suspects the dog has died. Two 
remain at his clinic getting "recumbent care to make sure they don't 
get bed sores" or secondary infections. One is a Cane Corso, a huge 
dog in the mastiff family. Kreissler said the dog weighed 110 pounds 
in September 2009 and weighs 70 now due to muscle wasting. The dog 
had complications and spent a day on a ventilator. Care so far is in 
5 figures, he said.

Still, Kreissler isn't sure he's dealing with botulism or that 
reptiles are the culprit. "It would be really convenient to blame the 
iguanas, but it might be premature," he said. With botulism, he'd 
expect to see problems with the autonomic nervous system that 
controls involuntary functions like pupil dilation and heart rate, 
"and I'm not seeing any of that. These dogs are alert and can wag 
their tails." Still, in analyzing the cases, vets have to take into 
consideration recent, abnormal environmental changes, such as a 
prolonged freeze that kills cold-blooded reptiles, he said.

--
Communicated by:
ProMED-mail <promed@promedmail.org>

[There is a useful video attached to the above article in which 
Sherry L. Schlueter, Executive Director of the Wildlife Care Center 
in Ft. Lauderdale, explains how to handle a frozen iguana. With 
temperatures dipping near freezing, these non-native reptiles can't 
handle the cold, and their scaly bodies shut down until they are able 
to warm up again.

_Clostridium botulinum_ grows in an anaerobic environment, and some 
of these iguanas will be quite large; during the cold snap there were 
various national TV news reports of chilled immobile iguanas falling 
out of the trees. Botulism, especially in animals, is a rare but 
serious paralytic condition caused by a nerve toxin produced by the 
bacterium _Cl. botulinum_. This organism produces a pre-formed toxin 
responsible for the nerve paralysis. The toxin binds to receptors on 
presynaptic terminals of cholinergic synapses, is internalized into 
vesicles, and then is translocated to the cytosol. In the cytosol, 
the toxin mediates the proteolysis of components of the 
calcium-induced exocytosis apparatus (the SNARE proteins) to 
interfere with acetylcholine release. Blockade of neurotransmitter 
release at the terminal is permanent, and recovery only occurs when 
the axon sprouts a new terminal to replace the toxin-damaged one. The 
classic syndrome of botulism is a symmetrical, descending motor 
paralysis in an alert patient, with no sensory deficits. If this is 
botulism, the animals would go down, and would normally die over a 
few hours as the muscles of respiration become paralyzed. 
Fortunately, for people and companion animals, there is an antitoxin. 
If there was a record of a successful canine recovery following the 
use of the antitoxin, it would add weight to this working diagnosis 
of botulism.

Anyone with direct information on these canine cases is asked to 
share it. If it is botulism, it would be nice to have laboratory 
confirmation of some sort.

More extensive information on botulism can be found at: 
<http://emedicine.medscape.com/article/829125-overview>. - Mod.MHJ]

[see also:
2009
----
Botulism, avian - USA: (NV) 20090808.2814
Botulism, equine - USA: (WY) 20090502.1651
2008
----
Botulism, avian - USA (02): Great Lakes 20080817.2556
Botulism, avian - USA: Great Lakes 20080116.0210
2007
----
Botulism, avian - USA (02): (MI), susp. 20071111.3665
Botulism, avian - USA: (MI), susp 20071103.3572
Botulism, avian - USA (NY): susp 20070812.2624
Botulism, avian - USA (NY) 20070714.2259]
..............................mhj/msp/mpp

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